ZEB1 Antibody

• Catalog Number: orb1240299
• Category: Antibodies
• Description: ZEB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: ZEB1 antibody was raised against a 15 amino acid synthetic peptide near the center of human ZEB1.The immunogen is located within amino acids 530 - 580 of ZEB1.
• Concentration: 1 mg/mL
• Dilution range: ZEB1 antibody can be used for detection of ZEB1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 124 kDa Observed: 130 kDa
• Target: ZEB1
• UniProt ID: P37275
• NCBI: NP_110378
• Storage: ZEB1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: ZEB1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ZEB1 Antibody: BZP, TCF8, AREB6, FECD6, NIL2A, PPC
• Note: For research use only
• Application notes: ZEB1 antibody can be used for detection of ZEB1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ZEB1 in HeLa cell lysate with ZEB1 antibody at 1 µg/mL.

Immunohistochemistry of ZEB1 in human liver tissue with ZEB1 antibody at 5 µg/mL.

Immunofluorescence of ZEB1 in Hela cells with ZEB1 antibody at 20 µg/mL.

Immunocytochemistry of ZEB1 in HeLa cells with ZEB1 antibody at 20 µg/mL.

Immunocytochemistry of ZEB1 in K562 cells with ZEB1 antibody at 2.5 µg/ml.