ZEB1 Antibody

SKU: orb1240299

Description

ZEB1 Antibody

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  ZEB1 antibody can be used for detection of ZEB1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: ZEB1 antibody was raised against a 15 amino acid synthetic peptide near the center of human ZEB1.The immunogen is located within amino acids 530 - 580 of ZEB1.
• Target: ZEB1
• Molecular Weight: Predicted: 124 kDa Observed: 130 kDa
• Purification: ZEB1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: ZEB1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ZEB1 Antibody: BZP, TCF8, AREB6, FECD6, NIL2A, PPCD3, ZFHEP, ZFHX1A, DELTAEF1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, TCF-8

Western blot analysis of ZEB1 in HeLa cell lysate with ZEB1 antibody at 1 µg/mL.

Immunohistochemistry of ZEB1 in human liver tissue with ZEB1 antibody at 5 µg/mL.

Immunofluorescence of ZEB1 in Hela cells with ZEB1 antibody at 20 µg/mL.

Immunocytochemistry of ZEB1 in HeLa cells with ZEB1 antibody at 20 µg/mL.

Immunocytochemistry of ZEB1 in K562 cells with ZEB1 antibody at 2.5 µg/ml.

Quick Database Links

Gene Symbol

ZEB1

UniProt

P37275

RefSeq (Protein):NP_110378