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Item 1 of 7
ZC3H12A (CT) Antibody
Catalog Number: orb1672348
| Catalog Number | orb1672348 |
|---|---|
| Category | Antibodies |
| Description | ZC3H12A (CT) Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC, WB |
| Predicted Reactivity | Gallus, Monkey, Porcine, Primate |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Anti-ZC3H12A antibody (orb1672348) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human ZC3H12A. The immunogen is located within the last 50 amino acids of ZC3H12A. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/mL |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Predicted: 66 kD Observed: 66 kD |
| Target | ZC3H12A |
| UniProt ID | Q5D1E8 |
| NCBI | Q5D1E7.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | ZC3H12A Antibody is supplied in PBS containing 0.02% sodium azide. |
| Alternative names | ZC3H12A Antibody: Endoribonuclease ZC3H12A, Monocy Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
WB Validation in Human Lung. Loading: 15 µg of lysate per lane. Antibodies: ZC3H12A orb1672348, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 2 µg/mL. Lane 2: 4 µg/mL.
Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: ZC3H12A orb1672348, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: ZC3H12A orb1672348, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of ZC3H12A in Human THP-1 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed THP-1 cells labeling ZC3H12A with orb1672348 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry Validation of ZC3H12A in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ZC3H12A antibody (orb1672348) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of ZC3H12A in Mouse Liver. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ZC3H12A antibody (orb1672348) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of ZC3H12A in Rat Brain. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody (orb1672348) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
