ZAP70 Antibody

SKU: orb534196

Description

Tyrosine-protein kinase ZAP70 is a tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, it phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. [UniProt]

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: FACS, IHC-P
• Dilution range: Flow cytometry: 1-2ug/10^6 cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The stated application concentrations are suggested starting points. Titration of the ZAP70 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: ZPT70-2
• Immunogen: A portion of amino acids 247-382 from the human protein was used as the immunogen for the ZAP70 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human tonsil with ZAP70 antibody (clone ZPT70-2). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Flow testing of PFA-fixed Jurkat cells with ZAP70 antibody (clone ZPT70-2); Red = isotype control, Blue = ZAP70 antibody.

Quick Database Links

UniProt

P43403