YY1 Antibody

SKU: orb1788301

Description

Mouse Monoclonal Antibody (Mab)

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1:1000, IHC: 1:25, IHC: 1:25, IHC: 1:25
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Immunogen: Purified His-tagged YY1 protein was used to produced this monoclonal antibody. Antigen Region: 1-404 aa.
• Target: YY1
• Molecular Weight: 44713 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

YY1;INO80S; Transcriptional repressor protein YY1; Transcriptional repressor protein YY1; Delta transcription factor; Transcriptional repressor protein YY1; INO80 complex subunit S; Transcriptional repressor protein YY1; NF-E1; Transcriptional repressor protein YY1; Yin and yang 1

Western blot analysis of lysates from Daudi, U-251 MG cell line (from left to right), using YY1 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Staining YY1 in Rat brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining YY1 in Monkey brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining YY1 in Zebra fish brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

YY1

UniProt

P25490

RefSeq (Protein):NP_003394.1