YY1 Antibody

SKU: orb166907

Description

Mouse monoclonal antibody to YY1.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:500-1:1000, IHC-P - 1:25
• Reactivity: Human, Monkey, Rat, Zebrafish

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 1183CT6.5.23.6
• Immunogen: Recombinant Protein
• Target: YY1
• Molecular Weight: 44713
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti YY1 antibody, anti INO80S antibody, anti Transcriptional repressor protein YY1 antibody, anti Transcriptional repressor protein YY1 antibody, anti Delta transcription factor antibody, anti Transcriptional repressor protein YY1 antibody, anti INO80 complex subunit S antibody, anti Transcriptional repressor protein YY1 antibody, anti NF-E1 antibody, anti Transcriptional repressor protein YY1 antibody, anti Yin and yang 1 antibody

YY1 Antibody western blot analysis in Daudi and U251 cell line lysates (35μg/lane). This demonstrates the YY1 antibody detected the YY1 protein (arrow).

All lanes: Anti-YY1 Antibody (Center) at 1:500-1:1000 dilution. Lane 1: 293 whole cell lysate. Lane 2: C6 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: U-251 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining YY1 in Rat brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining YY1 in Monkey brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining YY1 in Zebra fish brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

YY1

UniProt

P25490

RefSeq (Protein):NP_003394.1

Fernando, Thilini R. et al. The lncRNA CASC15 regulates SOX4 expression in RUNX1-rearranged acute leukemia Mol Cancer, 16, 126 (2017)

PubMed: 28724437