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Catalog Number | orb1931586 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Target | This YY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-104 amino acids from the N-terminal region of human YY1. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
UniProt ID | P25490 |
MW | 44713 Da |
Tested applications | WB |
Dilution range | WB: 1:2000, WB: 1:1000 |
Clone Number | RB2943 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Transcriptional repressor protein YY1, Delta trans Read more... |
Note | For research use only |
NCBI | NP_003394.1 |
YY1 Antibody (L89) western blot analysis in 293, HL-60, SiHa, K562, THP-1, U266 cell line lysates (35 ug/lane). This demonstrates the YY1 antibody detected the YY1 protein (arrow).
All lanes: Anti-YY1 Antibody (N-term) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: C2C12 whole cell lysate. Lane 3: C6 whole cell lysate. Lane 4: Daudi whole cell lysate. Lane 5: U-251 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |