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Catalog Number | orb1788422 |
---|---|
Category | Antibodies |
Description | Purified Mouse Monoclonal Antibody (Mab) |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | IF, WB |
Predicted Reactivity | Sheep |
Reactivity | Human, Mouse, Rat |
Isotype | IgG2b,κ |
Immunogen | 1-261 aa |
Antibody Type | Primary Antibody |
Dilution range | IF: 0.059027777777778, WB: 1:1000 |
Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 27745 Da |
Target | This YWHAZ antibody is generated from a mouse immunized with a recombinant protein from human YWHAZ. |
UniProt ID | P63104 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | 14-3-3 protein zeta/delta, Protein kinase C inhibi Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Fluorescent image of U251 cells stained with YWHAZ Antibody. Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from A431, HepG2, mouse NIH/3T3 cell line, mouse brain tissue, rat C6, PC-12 cell line (from left to right), using YWHAZ Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
ELISA, FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ICC, IF, IHC-P, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-Fr, IHC-P, WB | |
Drosophila, Sheep, Yeast | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |