YWHAG Antibody (N-term) [Knockout Validated]

SKU: orb1787817

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:500-1:2000, IHC-P: 1:10~50, FC: 1:10~50
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Other, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: 63-92 aa
• Target: This YWHAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human YWHAG.
• Molecular Weight: 28303 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

14-3-3 protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein gamma, N-terminally processed, YWHAG

YWHAG Antibody (N-term) western blot analysis in A431, Hela cell line, mouse brain and rat brain lysates (35 ug/lane). This demonstrates the YWHAG antibody detected the YWHAG protein (arrow).

YWHAG Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with YWHAG Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Predominant 28.7 kDa band for the HEK293T wild type lysate was observed at (3 ug/ml anti-YWAG) that matches the predicted size of 28.3 kDa. A less prominent 84.9 kDa band was also observed, which could be an aggregated/multimeric form of the target protein, a post-translationally modified form of the target protein, a splice-variant form of the target protein, or an unrelated protein which shares the antibody-reactive epitope. Significant reactivity were observed for bands at 28.3 and 84.9 kDa in the knock out lysate, suggesting incomplete knockout of the target gene.

Quick Database Links

Gene Symbol

This YWHAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human YWHAG.

UniProt

P61981

RefSeq (Protein):NP_036611.2