YBX1 Antibody (Center)

• Catalog Number: orb1931989
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This YBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-178 amino acids from the Central region of human YBX1.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rabbit, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P67809
• MW: 35924 Da
• Tested applications: IF, WB
• Dilution range: IF: 1:10~50, WB: 1:1000
• Clone Number: RB41913
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Nuclease-sensitive element-binding protein 1, CCAA
• Note: For research use only
• NCBI: NP_004550.2

Fluorescent image of A549 cell stained with YBX1 Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). YBX1 immunoreactivity is localized to Cytoplasm significantly.

YBX1 Antibody (Center) western blot analysis in 293T cell line lysates (35 ug/lane). This demonstrates the YBX1 antibody detected the YBX1 protein (arrow).