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Item 1 of 3
YBX1 Antibody (C-term)
Catalog Number: orb1930107
| Catalog Number | orb1930107 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This YBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-305 amino acids from the C-terminal region of human YBX1. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Other, Rat |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| UniProt ID | P67809 |
| MW | 35924 Da |
| Tested applications | IF, WB |
| Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000 |
| Antibody Type | Primary Antibody |
| Clone Number | RB14114 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Nuclease-sensitive element-binding protein 1, CCAA Read more... |
| Note | For research use only |
| NCBI | NP_004550.2 |
Fluorescent confocal image of U251 cell stained with YBX1 Antibody (C-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). YBX1 immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis of YBX1 (arrow) using rabbit polyclonal YBX1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2).
Western blot analysis of lysate from U-251 MG cell line, using YBX1 Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.
