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Catalog Number | orb1930108 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB14116 |
Tested applications | WB |
Predicted Reactivity | Mouse, Other, Rabbit, Rat, Zebrafish |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 35924 Da |
Target | This YB1 (YBX1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human YB1 (YBX1). |
UniProt ID | P67809 |
NCBI | NP_004550.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Nuclease-sensitive element-binding protein 1, CCAA Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of YBX1 (arrow) using rabbit polyclonal YBX1 Antibody (N-term) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2).
Anti-YBX1 Antibody (N-term) at 1:1000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.