YAP/YAP1 Antibody

SKU: orb1538338

Description

YAP/YAP1 Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, WB
• Dilution Range: ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), WB (1 - 5 µg/ml)
• Reactivity: Human
• Application Notes:
  Further information: Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (30 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone No.: 2F12
• Immunogen: YAP1 (NP_006097, 53 aa ~ 162 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Target: YAP / YAP1
• Purification: Purified from ascites by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

YAP1, 65 kDa Yes-associated protein, YAP, Yes-associated protein beta, Yes-associated protein delta, Yorkie homolog, YAP2, Yes-associated protein 1, Yes-associated protein 2, YKI, YAP65

Immunofluorescence of monoclonal antibody to YAP1 on HeLa cell (antibody concentration 30 ug/ml).

Western blot of YAP1 expression in transfected 293T cell line by YAP1 monoclonal antibody clone 2F12.

Detection limit for recombinant GST tagged YAP1 is approximately 0.03 ng/ml as a capture antibody.

Quick Database Links

Gene Symbol

YAP / YAP1