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Catalog Number | orb1274561 |
---|---|
Category | Antibodies |
Description | YAP1 Antibody, KO Validated |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 72kDa |
Target | YAP1 |
UniProt ID | P46937 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | YAP1, YAP, YAP2, YAP65, YKI Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:20 - 1:50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using YAP1 antibody at 1:1000 dilution.
Western blot analysis of extracts from normal (control) and YAP1 knockout (KO) HeLa cells, using YAP1 antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human placenta using YAP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using YAP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using YAP1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using YAP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using YAP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using YAP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using YAP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.