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YAP (phospho Ser127) rabbit pAb

Catalog Number: orb764364

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb764364
CategoryAntibodies
DescriptionRabbit polyclonal antibody to YAP (phospho-Ser127)
ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Monkey, Mouse, Rat
Concentration1 mg/ml
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
ImmunogenThe antiserum was produced against synthesized peptide derived from human YAP around the phosphorylation site of Ser127. AA range:93-142
MW65
Tested applicationsELISA, IF, IHC-P, WB
Dilution rangeIF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesAnti-YAP1 antibody, anti-YAP65 antibody, anti-York
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NoteFor research use only
YAP (phospho Ser127) rabbit pAb

Immunofluorescence analysis of Hela cell. 1, YAP (phospho Ser127) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). β-Tubulin Monoclonal Antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

YAP (phospho Ser127) rabbit pAb

Immunofluorescence analysis of rat-heart tissue. 1, YAP (phospho Ser127) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

YAP (phospho Ser127) rabbit pAb

Immunofluorescence analysis of rat-heart tissue. 1, YAP (phospho Ser127) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

YAP (phospho Ser127) rabbit pAb

Western Blot analysis of various cells using Phospho-YAP (S127) Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).