Y16

SKU: orb1304072

Description

Y16 is a potent and specific inhibitor of the Rho GEF LARG, binding with a Kd of 76 nM to block the LARG-RhoA interaction. This inhibition prevents RhoA activation and its downstream signaling pathways. Its utility, demonstrated in vitro, makes it a valuable chemical probe for researching RhoA-mediated cellular processes such as cytoskeletal dynamics and cancer cell invasion.

Research Area

Cell Biology, Pharmacology & Drug Discovery, Signal Transduction

Key Properties

• CAS Number: 429653-73-6
• MW: 384.43
• Purity: >99.99% (May vary between batches)
• Formula: C₂₄H₂₀N₂O₃
• SMILES: Cc1cccc(COc2cccc(\C=C3/C(=O)NN(C3=O)c3ccccc3)c2)c1
• Target: Ras,Rho
• Solubility: DMSO:10 mg/mL (26.01 mM);10% DMSO+90% Corn Oil:1 mg/mL (2.6 mM)

Bioactivity

• Target IC50:
  LARG:76 nM (Kd)
• In Vitro:
  METHODS: MCF7 breast cells were treated with Y16 (5, 10, 30, 50 μM), and the effects of Y16 on growth, migration, and invasion, as well as mammosphere formation, breast cancer cell activity, and characteristics related to tumorigenic potential were studied. RESULTS Y16 significantly inhibited the growth, migration, and invasion of MCF7 breast cancer cells; Y16-treated MCF7 cells also produced smaller size and dose-dependently reduced mammary X-sphere numbers.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, Ras, Y16, Y-16, Y 16, Rho GEFs

Compound Identifiers

PubChem CID

989521