Y16

SKU: orb1222266

Description

Y16, also known as RhoA-IN-Y16, is a RhoA inhibitor, Y16 blocks the binding of LARG, a DBL-family Rho guanine nucleotide exchange factor, with Rho (Kd = 80 nM). Y16 specifically inhibits LARG catalyzed activation of RhoA and RhoA signaling pathways. Y16 blocks the growth and migration of MCF7 breast cancer cells.

Key Properties

• CAS Number: 429653-73-6
• MW: 384.43
• Purity: >98% (HPLC)
• Formula: C₂₄H₂₀N₂O₃
• SMILES: Cc1cc(ccc1)COc1cccc(c1)C=C1C(=O)NN(C1=O)c1ccccc1
• Target: mTOR
• Solubility: DMSO : 25 mg/mL 65.03 mM;H2O : < 0.1 mg/mL

Bioactivity

• In Vitro:
  Y16 (10-30 μΜ; 24 hours; NIH 3T3 cells) could inhibit RhoA-GTP formation induced by serum dose dependently and is specific for RhoA. Y16 (10-30 μΜ; 24 hours; NIH 3T3 cells) efficiently inhibits serum or SDF-1α-induced phospho-MLC and phospho-FAK formation, which are downstream of RhoA. Cell Viability Assay Cell line: NIH 3T3 cells. Concentration: 10 μΜ, 30 μΜ. Incubation time: 24 hours. Result: Inhibited RhoA-GTP formation induced by serum dose dependently and was specific for RhoA. Western blot analysis. Cell line: NIH 3T3 cells. Concentration: 10 μΜ, 30 μΜ. Incubation time: 24 hours. Result: Inhibited serum or SDF-1α-induced phospho-MLC and phospho-FAK formation, which were downstream of RhoA.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RhoA-IN-Y16 | Y16

Quick Database Links

Gene Symbol

mTOR