XPNPEP3 Rabbit Polyclonal Antibody

SKU: orb2953119

Description

Anti-XPNPEP3 Polyclonal Antibodyis a rabbit polyclonal antibody detecting Xaa-Pro aminopeptidase 3 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat. Highlights Affinity Purified: Minimal background and high purity for reliable results. Multi-Application: Validated across multiple applications. Multi-Species: Cross-reactive for translational research.

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: E. coli - derived recombinant Human XPNPEP3 (Glu253-Gln482).
• Purification: Purified by antigen affinity column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Xaa-Pro aminopeptidase 3, APP3, XPNPEP3, Aminopeptidase P3, X-Pro aminopeptidase 3

A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting XPNPEP3 (orb2953119) at a concentration of 41.5 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of XPNPEP3 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

Western blot analysis was performed using anti-XPNPEP3 polyclonal antibody at 1 µg/mL on various samples. Lane 1: 293T cell lysate, Lane 2: HepG2 cell lysate, Lane 3: PC3 cell lysate, Lane 4: Mouse kidney lysate, Lane 5: Mouse heart lysate.

Quick Database Links

UniProt

Q9NQH7