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Catalog Number | orb1931869 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB2930 |
Tested applications | IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:2000, IHC-P: 1:50~100 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 35213 Da |
Target | This XLKD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-77 amino acids from the N-terminal region of human XLKD1. |
UniProt ID | Q9Y5Y7 |
NCBI | NP_006682.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Lymphatic vessel endothelial hyaluronic acid recep Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The anti-XLKD1 N-term Pab is used in Western blot to detect XLKD1 in mouse liver tissue lysate.
XLKD1 Antibody (N-term) western blot analysis in Y79 cell line lysates (35 ug/lane). This demonstrates the XLKD1 antibody detected the XLKD1 protein (arrow).
Western blot analysis of XLKD1 (arrow) using rabbit polyclonal XLKD1 Antibody (C61). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XLKD1 gene.
Anti-XLKD1 Antibody (N-term) at 1:2000 dilution + human liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.