XDH Antibody (N-term)

SKU: orb1928008

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB23202
• Immunogen: This XDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-234 amino acids from the N-terminal region of human XDH. Antigen Region: 206-234 aa.
• Target: XDH
• Molecular Weight: 146424 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Xanthine dehydrogenase/oxidase, Xanthine dehydrogenase, XD, Xanthine oxidase, XO, Xanthine oxidoreductase, XOR, XDH, XDHA

Western blot analysis of XDH Antibody (N-term) in mouse lung tissue lysates (35 ug/lane). XDH (arrow) was detected using the purified Pab.

XDH Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-XDH Antibody (N-term) at 1:1000 dilution + human fetal liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 146 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with XDH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

XDH

UniProt

P47989

RefSeq (Protein):NP_000370.2