XBP-1 Antibody

• Catalog Number: orb1240297
• Category: Antibodies
• Description: XBP-1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-XBP-1 antibody (orb1240297) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human XBP-1. The immunogen is located within amino acids 40 - 90 of XBP-1.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 28/40kDObserved: 28/40 kD
• Target: XBP1
• UniProt ID: P17861
• NCBI: P17861
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: XBP-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: XBP-1 Antibody: XBP2, TREB5, XBP-1, XBP2, X-box-bi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence Validation of XBP-1 in HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling XBP-1 with orb1240297 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

XBP-1 KO Validation in 293T Cells. Loading: 10 µg of lysate Antibodies: XBP-1 orb1240297, 4 µg/mL and beta-actin, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with Recombinant Protein. Loading: 100 ng of human XBP-1 recombinant protein per lane. Antibodies: XBP-1 orb1240297 (A: 0.5 µg/mL, B: 1 µg/mL and C: 2 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 23kD.

Western Blot Validation of XBP-1 in HepG2 Cells. Loading: 15 µg of HepG2 cell lysate Antibodies: XBP-1 orb1240297, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane1: 1 µg/mL Lane2: 2 µg/mL.

Western Blot Validation in Mouse Pancreas Tissue. Loading: 15 µg of lysates per lane. Antibodies: XBP-1, orb1240297 (4 µg /mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Liver Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: XBP-1 orb1240297 (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. (1) the absence and (2) the presence of blocking peptide.

Immunofluorescence Validation of XBP-1 in Human Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling XBP-1 with orb1240297 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of XBP-1 in Human Liver Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Liver Tissue labeling XBP-1 with orb1240297 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of XBP-1 in Mouse Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling XBP-1 with orb1240297 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry Validation of XBP-1 in Human Liver Tissue. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-XBP-1 antibody (orb1240297) at 1 µg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of XBP-1 in Mouse Spleen Tissue. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-XBP-1 antibody (orb1240297) at 1 µg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of XBP-1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-XBP-1 antibody (orb1240297) at 1 µg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.