WTIP Antibody (C-term)

• Catalog Number: orb1937916
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This WTIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids of human WTIP.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: A6NIX2
• MW: 45124 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:500, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB29854
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Wilms tumor protein 1-interacting protein, WT1-int
• Note: For research use only
• NCBI: NP_001073905.1

Anti-WTIP Antibody (C-term) at 1:500 dilution + Caco2 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of WTIP Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

WTIP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of WTIP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.