WST-1

SKU: orb1705839

Description

WST-1 is a water-soluble tetrazolium salt utilized in cell viability and proliferation assays, where it is reduced by mitochondrial dehydrogenases to a soluble formazan dye. It is widely applied in vitro for cytotoxicity screening, drug discovery, and measuring superoxide dismutase activity in biochemical research.

Key Properties

• CAS Number: 150849-52-8
• MW: 651.34
• Purity: 99.32%
• Formula: C₁₉H₁₁IN₅NaO₈S₂
• SMILES: O=[N+]([O-])C1=CC=C([N+]2=NC(C3=C(S(=O)([O-])=O)C=C(S(=O)([O-])=O)C=C3)=NN2C4=CC=C(C=C4)I)C=C1.[Na+]
• Target: Others
• Solubility: DMSO:250 mg/mL (383.82 mM);H2O:80 mg/mL (122.82 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:2.5 mg/mL (3.84 mM)

Bioactivity

• Cell Research:
  Instructions 1. Preparation of WST-1 solution: WST-1 powder is fully dissolved in DMSO to prepare a 10 mg/mL WST-1 stock solution. The prepared solution should be stored away from light, can be stored at 4℃ for one week, and can be stored at -20℃ for half a year, and avoid repeated freezing and thawing. 2. Cell inoculation: According to the purpose of the experiment, add an appropriate amount of cell suspension to a 96-well plate and culture overnight to ensure cell adhesion. Treat cells: According to the needs of the experiment, add different concentrations of the drug or stimulant to be tested to each well and continue to culture for an appropriate time. 3. Add WST-1 reagent: Add 5-10 μM WST-1 working solution to each well and mix gently. 4. Incubation: Place the culture plate in a cell culture incubator and incubate for 0.5 to 4 hours. The specific incubation time can be optimized according to the cell type and density, usually 1-2 hours. 5. Determine absorbance: Use a microplate reader to measure the absorbance of each well at a wavelength of 450nm and calibrate it with a reference wavelength of 600nm. The level of absorbance reflects the proliferation or survival of cells.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

WST 1, WST1, WST-1

Compound Identifiers

PubChem CID

6099081