WS6

SKU: orb1306467

Description

WS6 is a small molecule that induces β-cell proliferation by modulating EBP1 and the IκB kinase signaling pathway. It is a valuable research tool for studying pancreatic islet biology and diabetes mechanisms in both in vitro and in vivo experimental models.

Research Area

Cardiovascular Research, Neuroscience, Pharmacology & Drug Discovery, Signal Transduction

Key Properties

• CAS Number: 1421227-53-3
• MW: 568.59
• Purity: 99.95%
• Formula: C₂₉H₃₁F₃N₆O₃
• SMILES: C(C1=C(C(F)(F)F)C=C(NC(CC2=CC=C(OC=3C=C(NC(=O)C4CC4)N=CN3)C=C2)=O)C=C1)N5CCN(C)CC5
• Target: EGFR,IκB/IKK,FLT,PI3K,GABA Receptor
• Solubility: Ethanol:93 mg/mL (163.56 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (5.8 mM);DMSO:93 mg/mL (163.56 mM)

Bioactivity

• Target IC50:
  Proliferation (β cells):0.28 μM
• In Vivo:
  In R7T1 cells (EC50=0.28 μM), WS6 induces cell proliferation through the action on the Erb3 binding protein-1 and IκB kinase signaling pathways.
• In Vitro:
  In the RIP-DTA mouse model with β-cell ablation, oral administration of WS6 (50 mg/kg) promotes β-cell proliferation, thereby ameliorating diabetes.
• Cell Research:
  R7T1 cells are growth-arrested by removal of doxycycline for 2 days and plated into 384-well plates at a density of 3,000 cells/well in growth medium. Β cell proliferation is assessed using CellTiter Glo after 4 days. Fold increase in cell number is calculated by normalizing compound-treated wells to the median of DMSO-treated wells. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

WS 6, WS6, WS-6, IkB/IKK, IkB, IKK, IκB, IκB kinase, inhibit, Inhibitor, ErbB3-Binding Protein-1 (EBP2)

Compound Identifiers

PubChem CID

71566751