WS3

SKU: orb1306618

Description

WS3 is a small molecule that induces β-cell proliferation by modulating Erb3 binding protein-1 (EBP1) and the IκB kinase pathway. It is a valuable research tool for studying pancreatic islet biology, diabetes mechanisms, and regeneration in both in vitro and in vivo experimental models.

Research Area

Cardiovascular Research, Signal Transduction

Key Properties

• CAS Number: 1421227-52-2
• MW: 569.58
• Purity: 99.94% (May vary between batches)
• Formula: C₂₈H₃₀F₃N₇O₃
• SMILES: C(C1=C(C(F)(F)F)C=C(NC(NC2=CC=C(OC=3C=C(NC(=O)C4CC4)N=CN3)C=C2)=O)C=C1)N5CCN(C)CC5
• Target: IκB/IKK,Others,EGFR
• Solubility: DMSO:93 mg/mL (163.28 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:74 mg/mL (129.92 mM)

Bioactivity

• Target IC50:
  Proliferation (β cells):28 nM(ec50)
• In Vivo:
  In the mouse β-cell line R7T1, WS3 induces cell proliferation with an EC50 of 0.074 μM. Additionally, WS3 reversibly induces cell proliferation in primary embryonic and adult retinal pigment epithelial cells.
• Cell Research:
  R7T1 cells are growth-arrested by removal of doxycycline for 2 days and plated into 384-well plates at a density of 3,000 cells/well in growth medium. Β cell proliferation is assessed using CellTiter Glo after 4 days. Fold increase in cell number is calculated by normalizing compound-treated wells to the median of DMSO-treated wells. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

diabete, IκB, IκB kinase, inhibit, pancreatic, Inhibitor, insulin, IkB, IkB/IKK, IKK, ErbB3-Binding Protein-1 (EBP1), epithelial, R7T1, WS3, WS-3, WS 3, pigment, proliferation, RPE, retinal, β cell

Compound Identifiers

PubChem CID

72551586