SM22 Alpha/Transgelin Antibody

• Catalog Number: orb18630
• Category: Antibodies
• Description: Goat polyclonal to TAGLN
• Target: SM22 Alpha / Transgelin
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: MTGYGRPRQIIS
• RRID: AB_10750726
• MW: 22.6
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:4000, WB: 0.1-1 μg/ml, IHC-P: 2.5μg/ml
• Application notes: NP_003177.2 and NP_001001522.1 represent identical protein.ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 24kDa band observed in Human Placenta lysates (calculated MW of 22.6kDa according to NP_003177.2 and NP_001001522.1). Recommended concentration: 0.01-0.03 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti TAGLN antibody, anti SM22 antibody, anti SMCC
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 6876
• Expiration Date: 12 months from date of receipt.

orb18630 (0.03µg/ml) staining of Mouse Skeletal muscle lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

orb18630 (0.03µg/ml) staining of Human Skeletal muscle (A) and (0.003ug/ml) Testes (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemical staining of Human Prostate using TAGLN antibody

Western blot analysis of Human Testes lysate using TAGLN antibody