WNT9A Antibody

• Catalog Number: orb1256081
• Category: Antibodies
• Description: WNT9A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 206-365 of human WNT9A (NP_003386.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 30kDa
• Target: WNT9A
• UniProt ID: O14904
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Protein Wnt-9a, Protein Wnt-14, WNT9A, WNT14
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of mouse lung, using WNT9A antibody (orb1256081) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat lung using WNT9A antibody (orb1256081) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using WNT9A antibody (orb1256081) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using WNT9A antibody (orb1256081) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using WNT9A antibody (orb1256081) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using WNT9A antibody (orb1256081) at dilution of 1:100. Blue: DAPI for nuclear staining.