Wnt5a Rabbit mAb [KD Validated]

• Catalog Number: orb1951056
• Category: Antibodies
• Description: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression.
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Wnt5a
• Antibody Type: Recombinant Antibody
• Dilution range: WB 1:500~1:1000 ICC/IF 1:50~1:200 FC 1:20
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• UniProt ID: P41221
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: hWNT5A; Protein Wnt5a; Wingless type MMTV integrat
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of Wnt5a expression in HeLa cell lysate.