WNT5A Antibody

SKU: orb1538178

Description

WNT5A Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, WB
• Dilution Range: ELISA (1:10000), IF (1:200 - 1:1000), IHC, IHC-P (1:200 - 1:400), WB (1:100 - 1:500)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for This was determined to be 1:200.

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Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 6F2
• Immunogen: Purified recombinant fragment of WNT5A expressed in E. Coli.\
• Target: WNT5A
• Purification: Ascites
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Ascitic fluid, 0.03% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

WNT5A, HWNT5A, Protein Wnt-5a, WNT-5A protein

Quick Database Links

Gene Symbol

WNT5A