Wnt5a Antibody (HRP)

SKU: orb414048

Description

Rabbit polyclonal antibody against WNT5A conjugated to HRP

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB (1:1000); IHC (1:50)
• Reactivity: Human
• Application Notes:
  A 1:1000 dilution of SPC-737 was sufficient for detection of Wnt5a in 10 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of Human Wnt5a (200-300 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Target: Wnt5a
• Molecular Weight: 42.3 kDa, 35 kDa
• Purification: Peptide Affinity Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

WNT5A, Wnt Family Member 5A, Wingless-Type MMTV Integration Site Family Member 5A, WNT-5A, Protein Wnt-5a, HWNT5A, Epididymis Secretory Sperm Binding Protein

Immunohistochemistry analysis using Rabbit Anti-Wnt5a Polyclonal Antibody. Tissue: Thyroid Carcinoma. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Wnt5a Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~42.3 kDa Wnt5a protein using Rabbit Anti-Wnt5a Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Wnt5a Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~42.3 kDa. Other Band (s): ~35 kDa isoform 2.

Quick Database Links

Gene Symbol

Wnt5a

UniProt

P41221

NCBI Gene ID

7474

RefSeq (Protein):NP_001243034.1