Wnt5a Antibody: Biotin

• Catalog Number: orb414046
• Category: Antibodies
• Description: Rabbit polyclonal antibody against WNT5A conjugated to Biotin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human
• Immunogen: Synthetic peptide of Human Wnt5a (200-300 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); IHC (1:50)
• Conjugation: Biotin
• MW: 42.3 kDa, 35 kDa
• Target: Wnt5a
• Entrez: 7474
• UniProt ID: P41221
• NCBI: NP_001243034.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: WNT5A_HUMAN antibody, Protein Wnt 5a antibody, WNT
• Note: For research use only
• Application notes: A 1:1000 dilution of SPC-737 was sufficient for detection of Wnt5a in 10 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Rabbit Anti-Wnt5a Polyclonal Antibody. Tissue: Thyroid Carcinoma. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Wnt5a Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~42.3 kDa Wnt5a protein using Rabbit Anti-Wnt5a Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Wnt5a Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~42.3 kDa. Other Band (s): ~35 kDa isoform 2.