WNT3 Antibody

• Catalog Number: orb1538017
• Category: Antibodies
• Description: WNT3 Antibody
• Target: WNT3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Equine, Gallus, Human, Mammal, Monkey, Mouse, Porcine, Rat, Xenopus, Zebrafish
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Immunogen: Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:16000), WB (1 - 3 µg/ml)
• Application notes: Further information: Immunohistochemistry: LS-B3241 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3241 was determined to be 3.75 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: WNT3, Proto-oncogene Int-4 homolog, Proto-oncogene
• Note: For research use only

WNT3 antibody (1 µg/ml) staining of lysate of cell line HEK293 (35 µg protein in RIPA buffer). Detected by chemiluminescence.