WNT1 Antibody (C-term)

• Catalog Number: orb1929812
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19186
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 40982 Da
• Target: This WNT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-288 amino acids from the C-terminal region of human WNT1.
• UniProt ID: P04628
• NCBI: NP_005421.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

WNT1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

WNT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human testis followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WNT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of WNT1 Antibody (C-term) in mouse heart tissue lysates (35 ug/lane). WNT1 (arrow) was detected using the purified Pab.