WIF1 Antibody(Human C-term)

SKU: orb1931522

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P-Leica - 1:500
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD32706
• Immunogen: This WIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 347-376 amino acids from the C-terminal region of human WIF1. Antigen Region: 347-376 aa.
• Target: WIF1
• Molecular Weight: 41528 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Wnt inhibitory factor 1, WIF-1, WIF1

Immunohistochemical analysis of paraffin-embedded human colon tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Anti-WIF1 Antibody (Human C-term) at 1:1000 dilution + Mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-WIF1 Antibody (Human C-term) at 1:2000 dilution + Human skeletal muscle lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

WIF1

UniProt

Q9Y5W5

RefSeq (Protein):NP_009122.2