WEE1 Antibody (C-term)

SKU: orb1928601

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD5747
• Immunogen: This WEE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 604-634 amino acids from the C-terminal region of human WEE1. Antigen Region: 604-634 aa.
• Target: WEE1 {ECO:0000303|PubMed:8348613, ECO:0000312|HGNC:HGNC:12761}
• Molecular Weight: 71597 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Wee1-like protein kinase, WEE1hu, Wee1A kinase, WEE1

Anti-WEE1 Antibody (A619) at 1:1000 dilution + A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 72 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

WEE1 {ECO:0000303|PubMed:8348613, ECO:0000312|HGNC:HGNC:12761}

UniProt

P30291

RefSeq (Protein):NP_001137448.1, NP_003381.1