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WDR45 Antibody: Biotin
Catalog Number: orb384135
| Catalog Number | orb384135 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody against WDR45 conjugated to Biotin. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, ICC, IF, IHC, WB |
| Reactivity | Human, Rat |
| Immunogen | Synthetic peptide from the C-terminal of Human WIPI4 (aa. 312-322) |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000), ICC/IF (1:100) |
| Conjugation | Biotin |
| MW | 40 kDa |
| Target | WDR45 |
| Entrez | 11152 |
| UniProt ID | Q9Y484 |
| NCBI | NP_001025067.1 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS |
| Alternative names | WIPI4 antibody, WDR45 antibody, WDRX1 antibody, WD Read more... |
| Note | For research use only |
| Application notes | A 1:1000 dilution of SPC-651 was sufficient for detection of WIPI4 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDR45 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDR45 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WDR45 Antibody (D) Composite.
Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 40 kDa WDR45 protein using Rabbit Anti-WDR45 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDR45 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 40 kDa.
