WDFY3 Antibody: Biotin

• Catalog Number: orb384189
• Category: Antibodies
• Description: Rabbit polyclonal antibody against WDFY3 conjugated to Biotin.
• Target: WDFY3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Biotin
• Reactivity: Human, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Purification: Peptide Affinity Purified
• Immunogen: Synthetic peptide from the N-terminal of Human WDFY3 (aa. 533-544)
• UniProt ID: Q8IZQ1
• MW: 400 kDa
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Application notes: A 1:1000 dilution of SPC-666 was sufficient for detection of WDFY3 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: WDFY3 antibody, Alfy antibody, WD repeat and FYVE
• Note: For research use only
• Entrez: 23001
• NCBI: NP_055806.2

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDFY3 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) WDFY3 Antibody. (D) Composite.

Western blot analysis of Rat brain showing detection of 395.2 kDa WDFY3 protein using Rabbit Anti-WDFY3 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDFY3 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 395.2 kDa.