WAS Antibody

SKU: orb1248233

Description

WAS Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml. Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HepG2 and U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SPADKKRSGKKKI
• Target: WAS
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

WAS, WASP, Wiskott-Aldrich syndrome (eczema-thrombocytopenia), THC, IMD2, thrombocytopenia 1 (X-linked), Wiskott-Aldrich syndrome (eczema-thrombocytopenia) protein, THC1, Wiskott-Aldrich syndrome protein

orb1248233 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248233 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Quick Database Links

Gene Symbol

WAS

UniProt

P42768

RefSeq (Protein):NP_000368.1