VWF Antibody

SKU: orb1927145

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1：2000, IHC-P - 1:25, FC - 1:100
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: B129EV34X3X1
• Immunogen: This antibody is generated from a mouse immunized with .VWF recombinant protein.
• Target: VWF
• Molecular Weight: 309265 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

von Willebrand factor, vWF, von Willebrand antigen 2, von Willebrand antigen II, VWF, F8VWF

Anti-VWF at dilution + VWF whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 309 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Flow cytometric analysis of K562 cells using VWF (green) compared to an isotype control of mouse IgG1 (blue). Diluted at 1:100 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded H. kidney section using VWF. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Quick Database Links

Gene Symbol

VWF

UniProt

P04275