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Catalog Number | orb763376 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to VSV-G-Tag |
Clonality | Monoclonal |
Isotype | IgG1 |
Conjugation | Unconjugated |
Concentration | 1mg/mL |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
Immunogen | Synthetic Peptide |
Tested applications | WB |
Dilution range | WB 1:5,000 IP 1:200 IF 1:1,000 |
Source | Mouse |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
IP antibody use: 5ug VSV-G Mouse IgG1 per ml Lysate, WB 1:5000 1: untransfected 293 cell lysate 2: transfected 293 cell lysate with VSV-G-tag fusion protein 3: IP (untransfted 293 + anti-VSV-G mAb + Protein G agarose) 4: IP (transfected 293 + normal Mouse IgG + Pr).
IF analysis of 293T cells transfected with a VSV-G-tagged protein, using ELKbio VSV-G-Tag (8D6) Mouse mAb at a 1:2000 dilution (blue DAPI , red anti-VSV-G).
1ug VSV-G fusion protein + Primary antibody dilution at 1: 1:5, 000 2: 1:10, 000.