VSGP/F-spondin Antibody (N-term)

SKU: orb1937492

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cardiovascular Research, Neuroscience

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:2000
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD30914
• Immunogen: This VSGP/F-spondin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human VSGP/F-spondin. Antigen Region: 15-43 aa.
• Target: SPON1
• Molecular Weight: 90973 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Spondin-1, F-spondin, Vascular smooth muscle cell growth-promoting factor, SPON1, KIAA0762, VSGP

All lanes: Anti-VSGP/F-spondin Antibody (N-term) at 1:2000 dilution. Lane 1: Human heart lysate. Lane 2: Human lung lysate. Lane 3: Mouse lung lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 91 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

VSGP/F-spondin Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of VSGP/F-spondin Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

VSGP/F-spondin Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35 ug/lane). This demonstrates the VSGP/F-spondin antibody detected the VSGP/F-spondin protein (arrow).

Quick Database Links

Gene Symbol

SPON1

UniProt

Q9HCB6

RefSeq (Protein):NP_006099.2