VPS53 Antibody (N-term)

SKU: orb39233

Description

Purified rabbit polyclonal antibody.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB20279
• Immunogen: Synthetic Peptide
• Target: VPS53
• Molecular Weight: 94405
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Vacuolar protein sorting-associated protein 53 homolog antibody, anti-PP13624 antibody, anti-VPS53 antibody

Western blot analysis of VPS53 Antibody (N-term) in MCF-7 cell line lysates (35 ug/lane). VPS53 (arrow) was detected using the purified Pab.

Western blot analysis of VPS53 Antibody (N-term) in mouse brain tissue lysates (35 ug/lane). VPS53 (arrow) was detected using the purified Pab.

Flow cytometric analysis of MDA-MB468 cells using VPS53 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with VPS53 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

VPS53

UniProt

Q5VIR6

RefSeq (Protein):NP_001121631.1, NP_060759.2