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Catalog Number | orb1938599 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB25452 |
Tested applications | FC, IF, IHC-P, WB |
Predicted Reactivity | Mouse, Rat |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 82221 Da |
Target | This VPS52 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 610-637 amino acids from the C-terminal region of human VPS52. |
UniProt ID | Q8N1B4 |
NCBI | NP_072047.4 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Vacuolar protein sorting-associated protein 52 hom Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Confocal immunofluorescent analysis of VPS52 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
VPS52 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
VPS52 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VPS52 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
VPS52 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35 ug/lane). This demonstrates the VPS52 antibody detected the VPS52 protein (arrow).