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Description
Research Area
Neuroscience
Images & Validation
−Item 1 of 6
| Tested Applications | ICC, IHC-P, IP, WB |
|---|---|
| Dilution Range | WB: 1:1000-1:10000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary. |
| Target | VPS35 |
| Molecular Weight | 92 |
| Purity | Purified by antigen-affinity chromatography. |
| Purification | Purified by antigen-affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Buffer/Preservatives | PBS, 20% Glycerol, 0.025% ProClin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−VPS35 retromer complex component , MEM3 , PARK17
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Immunofluorescence analysis of HeLa cells using VPS35 antibody
Immunohistochemical staining of human colon carcinoma using VPS35 antibody
Western blot analysis of H1299(Lane 1), HeLa S3(Lane 2), Hep G2(Lane 3), MOLT4(Lane 4), Raji(Lane 5) using VPS35 antibody
Western blot analysis of H1299(Lane 1), HeLa S3(Lane 2), Hep G2(Lane 3), MOLT4(Lane 4), Raji(Lane 5) using VPS35 antibody
Western blot analysis of H1299(Lane 1), HeLa S3(Lane 2), Hep G2(Lane 3), MOLT4(Lane 4), Raji(Lane 5) using VPS35 antibody
Western blot analysis of H1299(Lane 1), HeLa S3(Lane 2), Hep G2(Lane 3), MOLT4(Lane 4), Raji(Lane 5) using VPS35 antibody
Quick Database Links
Gene Symbol
VPS35
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ICC
Immunocytochemistry
IP
Immunoprecipitation
VPS35 Rabbit Polyclonal Antibody (orb556484)
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