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| Catalog Number | orb556484 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to VPS35 |
| Target | VPS35 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | 0.025% ProClin 300. 1XPBS (pH7), 20% Glycerol |
| Purity | Purified by antigen-affinity chromatography. |
| Purification | Purified by antigen-affinity chromatography. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VPS35. The exact sequence is proprietary. |
| UniProt ID | Q96QK1 |
| MW | 92 |
| Tested applications | ICC, IHC-P, IP, WB |
| Dilution range | WB: 1:1000-1:10000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 |
| Application notes | Positive control: Mouse brain , rat brain , HeLa , 293T |
| Antibody Type | Primary Antibody |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles. |
| Alternative names | VPS35 retromer complex component , MEM3 , PARK17 |
| Research Area | Epigenetics, Infectious Diseases, Neuroscience |
| Note | For research use only |
![VPS35 antibody [C3], C-term](/images/pub/media/catalog/product/v/p/vps35-antibody_orb556484_ifa_1.jpg)
Immunofluorescence analysis of HeLa cells using VPS35 antibody
![VPS35 antibody [C3], C-term](/images/pub/media/catalog/product/v/p/vps35-antibody_orb556484_ihc_1.jpg)
Immunohistochemical staining of human colon carcinoma using VPS35 antibody
![VPS35 antibody [C3], C-term](/images/pub/media/catalog/product/v/p/vps35-antibody_orb556484_wb_1.jpg)
Western blot analysis of H1299(Lane 1), HeLa S3(Lane 2), Hep G2(Lane 3), MOLT4(Lane 4), Raji(Lane 5) using VPS35 antibody
![VPS35 antibody [C3], C-term](/images/pub/media/catalog/product/v/p/vps35-antibody_orb556484_wb_2.jpg)
Western blot analysis of H1299(Lane 1), HeLa S3(Lane 2), Hep G2(Lane 3), MOLT4(Lane 4), Raji(Lane 5) using VPS35 antibody
![VPS35 antibody [C3], C-term](/images/pub/media/catalog/product/v/p/vps35-antibody_orb556484_wb_3.jpg)
Western blot analysis of H1299(Lane 1), HeLa S3(Lane 2), Hep G2(Lane 3), MOLT4(Lane 4), Raji(Lane 5) using VPS35 antibody
![VPS35 antibody [C3], C-term](/images/pub/media/catalog/product/v/p/vps35-antibody_orb556484_wb_4.jpg)
Western blot analysis of H1299(Lane 1), HeLa S3(Lane 2), Hep G2(Lane 3), MOLT4(Lane 4), Raji(Lane 5) using VPS35 antibody
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