VPS28 Antibody

• Catalog Number: orb1246793
• Category: Antibodies
• Description: VPS28 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-ESAYNAFNRFLHA
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 24kDa band observed in H460, Human Testis and Brain lysates (predicted MW of 25kDa according to NP_057292.1). Recommended for use at 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Testis, stains acrosomal head caps in round spermatids. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: VPS28
• UniProt ID: Q9UK41
• NCBI: NP_057292.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: VPS28, vacuolar protein sorting 28 (yeast), vacuol
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 24kDa band observed in H460, Human Testis and Brain lysates (predicted MW of 25kDa according to NP_057292.1). Recommended for use at 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Testis, stains acrosomal head caps in round spermatids. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246793 staining (1 ug/ml) of Human Testis lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

orb1246793 (10 ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.