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| Catalog Number | orb770384 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to VPAC1 |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human VIPR1. AA range:332-381 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 52 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-VIPR1 antibody, anti-Vasoactive intestinal po Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of MCF7 cells, using VIPR1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HT-29 and K562 cells, using VIPR1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HeLa cells using VIPR1 antibody.
Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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