von Willebrand Factor / vWF antibody

SKU: orb2276455

Description

von Willebrand Factor / vWF antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:50-1:100
• Reactivity: Human
• Application Notes:
  Positive Control: Colon: A moderate to strong endothelial vWF immunostaining should be seen in a fraction of blood vessels. Negative Control: Colon: vWF immunostaining should be completely absent in epithelial and muscular cells. Cellular Localization: Cytoplasm Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit according to the manufacturer’s directions.

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Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-521R
• Immunogen: Recombinant fragment of human vWF protein (aa1815-1939) (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2,von Willebrand Disease (vWD)

Quick Database Links

UniProt

P04275