von Willebrand Factor / vWF Antibody [VWF/1465]

• Catalog Number: orb1252191
• Category: Antibodies
• Description: von Willebrand Factor / vWF Antibody [VWF/1465]
• Target: VWF
• Clonality: Monoclonal
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: Amino acids 1815-1939 from the human protein were used as the immunogen for this vWF antibody.
• UniProt ID: P04275
• Tested applications: FC, IF, IHC-P, IP, WB
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 minutes at RTWB: 0.5-1 ug/mlIP: 1-2ug/500ug lysateThe optimal dilution of the vWF antibody for each application should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: VWF/1465
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: VWF, Coagulation factor VIII VWF, F8VWF, VWD, Von
• Note: For research use only

Western blot testing of 1) partial recombinant protein and 2) human lung lysate with vWF antibody (clone VWF/1465). Predicted molecular weight ~250 kDa.

IHC testing of FFPE human tonsil with vWF antibody (clone VWF/1465). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free vWF Antibody (clone VWF/1465). Confirmation of Integrity and Purity of the Antibody.