VWF Antibody

SKU: orb1252191

Description

VWF Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, IF, IHC-P, IP, WB
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 1-2 µg/mL for 30 minutes at RTWB: 0.5-1 µg/mLIP: 1-2ug/500ug lysateThe optimal dilution of the vWF antibody for each application should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: VWF/1465
• Immunogen: Amino acids 1815-1939 from the human protein were used as the immunogen for this vWF antibody.
• Target: VWF
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

VWF, Coagulation factor VIII VWF, F8VWF, VWD, Von Willebrand factor

Western blot testing of 1) partial recombinant protein and 2) human lung lysate with vWF antibody (clone VWF/1465). Predicted molecular weight ~250 kDa.

IHC testing of FFPE human tonsil with vWF antibody (clone VWF/1465). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free vWF Antibody (clone VWF/1465). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

VWF

UniProt

P04275