von Willebrand Factor Antibody / vWF

• Catalog Number: orb750065
• Category: Antibodies
• Description: von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: VWF/1767
• Tested applications: ELISA, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Amino acids 1815-1939 from the human protein were used as the immunogen for this vWF antibody.
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This vWF antibody is available for research use only.
• UniProt ID: P04275
• Storage: Store the vWF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the vWF antibody for each application should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.