vWF Antibody / von Willebrand Factor

SKU: orb749396

Description

von Willebrand Factor is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This vWF antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi�s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, IP, WB
• Dilution Range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunoprecipitation: 1-2ug/500ug protein lysate,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the vWF antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: 3E2D10
• Immunogen: Amino acids 845-949 were used as the immunogen for this vWF antibody.
• Purification: Protein G purified vWF antibody
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of human tonsil with vWF antibody (clone 3E2D10). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.

IHC testing of FFPE human pancreas tissue with vWF antibody (clone 3E2D10). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.

SDS-PAGE analysis of purified, BSA-free vWF antibody (clone 3E2D10) as confirmation of integrity and purity.

Immunofluorescent staining of human brain tissue with vWF antibody (clone 3E2D10, green) and DAPI nuclear stain (blue).