von Willebrand Factor Antibody [SPM577]

• Catalog Number: orb1251869
• Category: Antibodies
• Description: von Willebrand Factor Antibody [SPM577]
• Target: VWF
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: A partial recombinant human protein was used as the immunogen for this anti-von Willebrand Factor antibody.
• UniProt ID: P04275
• Tested applications: FC, IF, IHC, IP, WB
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.5-1 ug/mlImmunoprecipitation: 0.5-1 ug/500ug protein lysateImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)The optimal dilution of the anti-von Willebrand Factor antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Antibody Type: Primary Antibody
• Clone Number: SPM577
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: VWF, Coagulation factor VIII VWF, F8VWF, VWD, Von
• Research Area: Cancer Biology
• Note: For research use only

Formalin-fixed, paraffin-embedded human tonsil stained with anti-von Willebrand Factor antibody (SPM577)