VWF Antibody

SKU: orb1251869

Description

VWF Antibody

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: FC, IF, IHC, IP, WB
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLWestern blot: 0.5-1 µg/mLImmunoprecipitation: 0.5-1 ug/500ug protein lysateImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)The optimal dilution of the anti-von Willebrand Factor antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: SPM577
• Immunogen: A partial recombinant human protein was used as the immunogen for this anti-von Willebrand Factor antibody.
• Target: VWF
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

VWF, Coagulation factor VIII VWF, F8VWF, VWD, Von Willebrand factor

Formalin-fixed, paraffin-embedded human tonsil stained with anti-von Willebrand Factor antibody (SPM577)

Quick Database Links

Gene Symbol

VWF

UniProt

P04275